Tuesday, April 23
Data, analysis and conclusion videos?
15 min only
Clean/organize for next lab
Review Purpose and Protocol for GFP Purification using HIC
GFP Pre-Lab due Thursday
questions, purpose, safety, protocol
Include Pictures, and leave a space to draw pictures
Tuesday, April 9
Peer Review, Lab write-up
Let's review our timeline for the rest of the year:
This week, Chapter 7 group presentation
4/16 - Lab 7.1 Protein Quantitation and the Bradford Assay
4/23 - Lab 7.2
4/30 - Lab 7.3
5/2 - BioTaPP Final/Practicum
Speed Presentations, 4-5 Google slides, about 5 min in class, starting at 10:15 Thursday
Proteins in Biotechnology (Raven/Emma)
(Masey and Jacob)
Homework: work on your slide presentation as needed
Revise your PCR Lab based on class discussions and peer reviews, due FRIDAY
Tuesday, April 2nd
Part 3 PV92 Lab - running our gels
Tuesday, March 26th
1. Read lab 6.3, PV92
2. Do the pre-lab for 6.3,
Parts 1A (skip the hair follicle)
You can write-up Part 3 this weekend
Tuesday, March 19th
and PCR Intro if time....
none tonight, enjoy...
(well, actually if you understood nothing about blue-white screening then you need to go back to ground zero and get some knowledge on this, as you will see it on an assessment....)
Tuesday, March 5th
Lab Write-Up discussion
Re-write any or all of your lab write-up as you see fit to get the grade you wish to get - due the Tuesday you return
Tuesday, February 26th
1. Get your Lab Notebooks, great job so far, and still room for improvement -
we need to revisit the analysis and conclusion
2. Did you do the pre-lab questions? Check this
3. Part 1 of our PGLO Lab: prepare our specialty plates (LB, LB/Amp, LB/Amp/Arabinose)
Full pre-lab write-up for 5.2
pre-lab questions, Purpose, safety materials, protocol (use many more graphics, far fewer words, make sure to highlight times)
No Lab Write-Up? No Lab.
Tuesday, February 19th
Check notes - graded, 10 pts
Check Terminology, graded 24 pts
Figures 5.4, 5.5 questions - graded10 pts
Transfer of genetic material in bacteria bacteria
Amoeba Sisters, Gene Regulation Video - take notes
Use the feedback from peers to amend your lab write-up, due Thursday
Watch the video on the Lac Operon - take notes
Tuesday, February 12th
Part 2 of 4.5 RFLP Lab today
Tuesday, February 5th
1. How did the NEBCutter site go?
Did you follow up in your lab notebook?
2. Assess your knowledge of this site
3. Peer Assessment of each other's labs
4. 4.5 Lab - Read pp. 126-7
5. Lab Prep
Full pre-lab write-up for 4.5 due Thursday
Make sure you are paying attention to what you are reading and writing, you will have a quiz on Thursday on the steps in the lab.
Thursday, April 25
Thursday April 11
Prepare for 7.1 that we will do on Tuesday
Thursday, April 4th
Discussion on transposable elements
Transposable Elements Video - no accent
Watch Video on PElements
Thursday, March 28th
Part 1A and Part 2 of Lab 6.3
Thursday, March 21st
Screening, quick review
Intro to PCR
Lab clean-up for gel pour tomorrow
Write up the calculations to make a 75 ml 3% agarose gel, 5 pts due Fri
Video on PCR
Video on PCR, watch and take notes as needed
Thursday, March 7th
Thursday, March 7th
CAREFULLY read pages 142 and 143, the section on selection of transformed cells - examine diagram 5.10 carefully.
Watch the videos on blue-white screening
Read the readings on blue-white screening:
Be prepared to explain competently and confidently the process of blue-white screening and the specifics of how it works!
Homework: Have your final lab write-up and complete and thorough understanding on blue-white screening. If you need more than what I have provided the internet is at your fingertips!
Thursday, February 28th
Homework: None :)
Thursday, February 21st
Get out your five questions regarding the LAC Operon and the BAD Operon (PGLO)
Little group activity
Let's take a look at the regulatory component of our PGLO System
Answer the pre-lab questions for 5.2 (PGLO Transformation Lab)
Review plate pouring as needed for Tuesday
If needed, Reading on the Lac Operon
Thursday, February 14th
Review our results, complete the formal lab write-up
Complete your formal lab write-up for a peer review, and Andrea review, by tomorrow
Thursday, February 7th
1. Little quiz on the protocol - what's happening - no book help on this
2. Lab 4.5 lab - Restriction digest (RFLP)
cast our gels
set up water baths
3. Plasmid mapping problem,
we will do this next week
Be absolutely 100% certain about what is happening tomorrow so we don't get popped by time.....
Friday, April 26
Conferences, no regular classes
Friday, April 12
Friday, April 5th
Lab write-up in class, if you are out make sure you have it done by next Tuesday
Friday, March 29th
Pour gels for our lab Monday
Friday, March 22nd
Gel Pour for 6.3
Read the 6.3 Lab
PV92 Gene reading
Alu insertion video - take notes!! You may use these on a quiz at some point
Friday, March 8th
No School, Enjoy Break!!!
Friday, March 1st
How did we do? Any gifts? I don't really need one, thank you....
Make sure you have your lab write-up by Tuesday.
Friday, February 22nd
Monday schedule, Gotcha!
Friday, February 15th
4.5 DNA Fingerprinting Lab
Formal Peer Review
1. Read and take notes (graded) in your lab notebook on pp. 134-142 (up to selection of transformed cells)
2. Also, define the bold and black terms, also neatly in your lab notebook (also graded)
3. Analyze the Figures 5.4 and 5.5 for discussion on Tuesday, write five questions you have about it (graded, again) in your lab notebook
4. Be ready for a quiz on the terms, make sure you have a true handle on the terms - I won't repeat the language in the text.
Friday, February 8th
Part I - restriction digests
This will be tight timing, so please make sure you are totally to be in the lab
Week of January 28th
Fragment size calculations? Let's review these, how was your line of best fit?
Look at 4.1 today as a class
Gel Electrophoresis Scripts for your video skills log - let's revisit these for videoing this week
Thursday (1/31) and Friday (2/1)
4.1 and Gel videoing
You will have a schedule for videoing in pairs of two and one group of three in the lab for Thursday and Friday. Be sure to:
wear ALL PPE, hair back coats buttoned up, no silly hats, etc
Gather all your materials AND the dummy gel and the dummy loading dye
Use the dummy gel and dummy loading to demonstrate how to set up the gel correctly and load ONE lane as demonstration of how to load properly
When you are finished break it all down for the next group, rinse the dummy gel with water and leave in the ziploc for next group.
Lab 4.1 - Formal Lab write-up to include: Purpose statement, pre-lab questions, protocol (I'll copy that for you),Results/Analysis as the book describes, and post-lab questions
Thursday, Jan 24th
Calculating the size of unknown frags using a standard
Tuesday, Jan. 22nd
Quick Kahoot DNA Review
Let's get some Flashcards going... terms:
Finish your flashcards (term on one side, def and pic on other - make sure to use color) by Thursday.
Okay, Plasmid Quiz......
Part is take home - DUE TOMORROW!!!!!
Decide if you are committed to being here next semester
Plasmid Mapping..... kind of challenging, so pay attention....
Be prepared for a quiz on the basics of plasmid mapping.....
Do the DNA Scissor Activity together in class
Look at our gel results!
Notes on how to determine unknown fragment size using the ladder
Lab Today - Gel Electrophoresis Lab, come ready to roll.
1. For the next quiz, start defining and examining the following terms and concepts. Make sure to ask yourself, and be prepared to sufficiently answer the question: what is the application of ______________, as well as other details related to the term/concept:
Little Quiz from Tuesday's notes
1. Read pp 105-111 (up to but not past Agarose Gel Staining and Imaging)
2. Define the following terms:
4. Last twenty minutes, discussion!!
Watch the video Part 2, Restriction enzymes, to the right
Watch the two video on Gel Electrophoresis
Take notes on these in your Lab Notebook - I will check this on next Tuesday
Study your terminology from class today
Notes on DNA
Review the Quest, get your work back from the basket
Study the notes from today for a quiz Thursday
Quiz on Gram staining and DNA
Movie on the historical events that led to the discovery of the structure of DNA (DRAMA!)
Gram Stain Lab,
Staph and Strep will stain purple because they are Gram + (thick cell wall)
Neisseria and E.coli will stain pink because they are Gram - (thin cell wall)
Know the examples above
Read pp. 102-105, by Thursday, and be prepared to demonstrate that you know:
The Central Dogma (Fig. 4.2)
Know the monomer of DNA is a nucleotide, know the three parts of a nucleotide
Know the basic structure (Figs. 4.5 and 4.6)
You will have a quiz Thursday on this material!
Watch the video on Gram Staining
Make sure you understand what a gram stain is for and the steps!!!
Do the pre-lab questions in your lab notebook
Write the protocol on p. 93-94 from Part 2
Copy your lab write-up and turn it in to me
Notes on gram staining on the board
Video on gram staining
Review material for the Quest Thursday
Read about bacteria structure and Gram Stain (next lab) in your text, Chapter 3,
And then read the Gram Staining Lab, this will be the next lab.
Get your PPE on and take turns videoing the analysis and conclusion for the Kirby-Bauer Lab (again), you can reference your official tables to compare your positive control to.....
If time, work on your write-up.....
Get your work back from the basket
Review your Titration Lab
Review what will be on the Quest 11/1
Moles versus molarity
What are the parts of anal/concl
Indictators - phenolphthalein (when is it pink, how did we use it)
Standard deviation - what does it tell us
how to hold it and use it properly
how to set/read it corectly
pay attention to its range and the hash marks
Labeling and pouring plates
Streaking a plate for a lawn
What was the purpose of the KB Lab, how to interpret ZOI's and tables
KB lab write-up due by Tuesday
Review the purpose statement!!
Peer review on lab write up for K-B Lab
Re-pour, let's see who can keep their plates sterile
In your lab notebook, in ink and written in your own hand (!),
Write out the steps of pouring plates as you understand it
1. Do not consult any other source
2. Brainstorm this on scratch paper BEFORE writing in your lab
3. Have this FINISHED and ready to share on Thursday at the
beginning of the period
**We will have a test following the Kirby-Bauer Lab on everything we have covered so far
Homework: see Thursday's homework
Continue pouring plates!
Go into your google folder that we share and please name your videos
Any voluntolds to share theirs with the class (you are all safe, we are learning together
Kirby Bauer write-up, due Tuesday
Pre-lab questions - due tomorrow, rest Tuesday
Let's pour some plates today!
6 pm, no homework
Standard Deviation! What does this mean, and why should we care?
Calculate the standard deviation for your titration trials
If you are totally lost go to the Biology Tab and scroll down to the short videos on standard deviation
Thursday , 10/4, posted Thurs 10/2, 5:30 pm
Homework: posted Fri, 10 am
1. If after our class today you think your video anal/conclusion need to be redone, please re-do them by Sat
Tuesday , 10/2, posted Mon 10/2, 8:30 am
Homework: posted Fri, 10 am
1. Do your video analysis and conclusion by Thursday
****Include an image of your data table and your calculations for the molarity of the unknown sodium hydroxide
2. Read pp. 58-62 only up to Uses of bacteria in biotechnology, take notes and pay great car on Koch's Postulates
Friday , 9/28, posted sat 9/28, 3:00 pm
Homework: posted Fri, 10 am
1. Do the pre-lab material before Tuesday:
Purpose/hypothesis, safety, materials, protocol
Thursday, 9/27, posted Wed 9/26
Tuesday, 9/24, posted Wed, oops....
Re-write your lab in one continuous document, due Thursday
Complete your post-lab questions as a part of your lab write-up (the last part)
Thursday, 9/20, posted 9:30 am Thurs
Re-do your purpose video, doing it at home is ok, re upload it to our folder
Tuesday, 9/18, posted 9 am Tues
Homework: posted Tues, 9:00 am
Friday, 9/14 (posted 3:45 pm Thurs)
I'm out today......
Copy down the notes from the board
Attempt 1-4 using my example for number 1,
Do your work on a separate piece of paper in case you mess up!!
Homework: posted at 2:20 pm Friday
A review from Biology, Polarity and Polar Molecules
Watch the Bozeman video to the right, take notes, this concept is the foundation for our first lab. Please keep a question list on things/vocab you don't understand
Secret Message Micropipetting Activity
Watch the videos to the right on Molar solutions by tomorrow,
take notes for a grade that I will check Monday
1. Homework, notes on Chapter 2......
2. Practice calculations for solution making
3. In the Lab,
Micropipette use and activity today!!
Assign lab partners, stations, equipment...
Collage gallery walk with peer feedback
Read CHapter 2, up to page 31, take notes
Welcome to BioTaPP! Tuesday, 9/4/18
Introduction: Biotechnology Collage, post to Digication when finished, and our shared Google Folder, 15 points
BioTaPP Syllabus, review
Get your texts!
Create your Lab Coat
Get your Syllabus and Safety Contract signed, 10 points
Finish your Biotechnology Collage, be prepared to share on Thursday