Monday 11/13/16
Transformation Lab Today In down time, peer review PCR Scripts Next Quiz: Thursday 11/9/16
Ligation component of our lab today, make sure your samples are in the freezer for Monday's transformation lab on Monday. Tuesday 11/7/17
Bold Systems Update: I did hear back from Alexandra at BOLD - she can only delete all traces for a sample, not selectively delete. I attempted to upload Amber and Zada's samples, and Lucie and Addy's as well. We can check on how to do that. She said she will call us, but I don't know when - it is her day off and I am not sure what time zone she is in..... Review the Ligation Experiment Protocol, this is a go for Thursday......!! Next videos, let's figure out a timeline and take a look at where we are at on these. If time, we will write/peer review scripts..... Monday 11/6/16
Okay, back on-line..... recovering from all that happened last week! Our new reagents have been ordered, and will be here Wednesday. Today, Sequetech and BOLD Database work - we have it figured out! Thursday, 10/26/17
Discuss grades and videos - feedback? Look at our enormous task of analyzing our fish barcoding sequences (how do you eat an elephant? One bite at a time!) Look at our plates...we have growth. Homework: Re-do any assingments, or start Review background materials on ligation, plasmids, transformation Review transformation protocol Wednesday, 10/25/17
Ligation Lab! Tuesday, 10/24/17
Review Plasmids, Monday, 10/23/17
Review DNA Barcoding Check our plates - we have some contamination Go over plasmids Lac Operon/IPTG Thursday, 10/19/17
Do you need some work time? Discuss plate making and how to store Peer review your work? Have you uploaded your other peer reviews into your BioTaPP folder? Overview of where we are at! Review our ligation lab protocol, we will be starting on this either Monday or Tuesday. On Tap: Fish Barcoding....... Wednesday, 10/18/17
1. Discuss how to pour plates, each group will pour 6 plates Store upside down on the counter 2. If time, polish your assignments due Thursday, 3. Please make sure you put these in you Google Folder that is shared with me I will pour your LB/amp/IPTG plates for you Friday Homework: work Tuesday, 10/17/17
Review work handed back Discuss 'Assignment Mitigation' Quizzes and questions, can do corrections up to 80% Videos, can do two retakes for full credit New groups! PCR Kleen today....store samples in your boxes Homework: work on your assignments due Thursday Monday, 10/16/17
Wow, do we have a lot to cover this week! And next....
Friday, 10/13/17
Check your three-ring binders Digication Update! Progress, yet not ultimate success, stay tuned. In the meantime, All BT students start a folder in Google Docs and put you required work in there so that when Digication is ready, you are ready (this is what we agreed on as a staff in our staff meeting) Take Stock of where you are at regarding your assignments due next: 1. Content Video Log 1 2. Your first formal lab write-up, please see the tab on lab write-up requirements 3. Your next set of Skills videos Work Time - 30 minutes Review the article I gave you yesterday Homework: Read the overview for your Ligation and transformation, Thursday, 10/12/17
Fishbarcoding samples have been received by Sequetech, now we wait for results. Video Skills Log - Amazing. Powerful. Impressive. I am going through these right now and I am wondering if we might have a few brave souls who would share theirs and allow a class critique? I think this would be very valuable...... At least, please do view two peers videos each, provide a written critique for each person to go into digication along with their video (and my feedback). Let's discuss this. Let's go over some questions - wobble versus degenerate primers, the same thing different name? What do you think? What are we doing? Let's take a step back and take a bird's eye view before we move forward. Questions/discussion. Last 30 min. of the period - I will be in the SS Mike with some of you for Digication training; you will start either 1) your next video skills log or 2) your content log on the lab you didn't write-up or 3) you will work on your formal lab write-up. You must be actively working on one of these things. Please share your work with Andrew and Jiren as they come around to see how you are learning/growing. Tuesday, 10/10/17
PCR Questions, turn these in to me please Due Date Discussion! Lab write-up Video Skills Log Video Content Log Review Components of Lab Write-Up Establish what labs of 1,2,3 you are writing up Work time in class for write-up or content logs, or skills logs Monday, 10/9/17
Yay, it's Monday!! All Day!! Review our results, discuss Analysis (this is hard) Friday, 10/6
Quiz - 50 points Thursday, 10/5
Run our gels to confirm product Quiz tomorrow Video 1 and 2 due tomorrow Homework: Questions on the lab, due Monday Tuesday and Wednesday, 10/3-10/4
Initial GAPDH PCR Nested GAPDH PCR Monday, 10/2/17
Good Morning! Regroup after our lab on Friday Discussion on our next lab, Nested PCR Some Power Point slides Pour Gels for Thursday - we will need more than 6 wells for this (let me check) Line out our schedule for the week Tuesday Initial PCR Wednesday Nested PCR Thursday Run confirmation gel and check results Friday - Quiz, as we discussed and also including PCR Homework: Prepare for lab tomorrow Read the entire packet Thursday/Friday 9/29-9/30
Prepare for our GAPDH extraction lab with our plants Assign groups/spreadsheet Plant Extraction Lab!! Wednesday, 9/28
Quiz next Friday, 10/6 (see quiz tab - website) First two skills videos on Micropipetting due next Friday Review the next Lab! How are we going to do plants? Every one do the same, or half the class share, etc? We need to get our plants in! Go over protocol..... It's go time on our next lab... tomorrow or Monday! Homework: Watch the video on glycolysis, note the GAPDH gene, and function Feel free to investigate further on-line if you wish Wednesday, 9/27
Go over our results, you all did great!! learn about the new Gel Doc EZ Clean up the BioTaPP Room Discuss quiz material - Date to be decided (I'm thinking 10/6) Get new lab reading.... Homework: Read through our flow for the GAPDH cloning (rest of the semester) Read through the longer reading (but easier) on DNA extraction from plant material (to harvest the GAPDH gene) Watch the video on Glycolysis by Thursday Tuesday, 9/26
Load our gels! Homework, Please read your packet on our labs Monday, 9/25
PCR Today on our samples Discuss PCR questions Tomorrow, Gel eletrophoresis on our samples, check them on the Gel Doc Homework: watch these videos Happy Friday! 9/22/17
Make and pour our gels for our PCR Lab Go Over the PCR protocol and PCR Theory Homework: PCR questions from last night's homework, in the button below, due Monday Thursday 9/21/17
Begin going over our formal lab write-up Purpose statement, write, and peer review for the DNA Fish Barcoding Lab Gel Electrophoresis! We will be pouring our gels tomorrow, so lets review this so we are ready Gel protocol for tomorrow Homework: Answer questions 1,2,3 by tomorrow (graded, type these up!) See button below Wednesday 9/20/17
Part I DNA Fish Barcoding Lab - Questions, review/self grade these LAB - Micropipette Practice
Formal lab write-up *Review the overall steps *Look at the purpose, and error Homework: Tuesday 9/19/17
Fish DNA Barcoding Lab, Part I: DNA Extraction In the lab performing protocol, part I Homework: Do the review questions for part I (1-4) Monday 9/18/17 Prepare for Lab tomorow go over protocol go into lab stations, set up drawers and lab materials TGIF, 9/1517. YAY!
Review cellular anatomy, 15 minutes Mitochondrial DNA! Reading on DNA Barcoding 15 minutes Lab Protocol - DNA Barcoding 30 minutes - over the weekend In the Lab, Micropipette review/practive Veterans lead! - next time! Make our handles - last 15-20 minutes Homework: 1. Start thinking your strategy for your video log review content review skills 2. Watch video son Polymerase Chain Reaction (PCR) (below) 3. New to BioTaPP, or just want to review Start checking the BioTaPP I page on the website for tutorials and reviews, I'll be posting to this regularly (starting tonight, you have got to give me until tonight :) 4. Read the reading below (Teacher Background) up to Global Initiatives Good Morning, Thursday, 9/14/17 Remember your Nitrile Gloves! Regroup - How are your projects? We are going to go through these one a day or so over the next few weeks Go over the grading rubric/materials you need Homework: Watch the link to the right on DNA barcoding Good Tuesday, September 5, 2017 Welcome to BioTaPP! First day, a collaboration and compilation of how biotechnology has been used! Discussion. Then, take a look at the new and exciting CRISPR Theory, and how we might see significant changes in the next few years! Homework: How would you use biotechnology to definitively identify a species? Discussion tomorrow. |
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